| 產品名稱 |
CL-S1 |
| 商品貨號 |
B164275 |
| Organism |
Mus musculus, mouse |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender |
female |
| Applications |
Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions |
liquid nitrogen vapor phase |
| Clinical Data |
female |
| Genes Expressed |
plasminogen activator (low amounts) |
| Cellular Products |
plasminogen activator (low amounts) |
| Comments |
Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 4.5 g/L glucose and 10 mcg/ml insulin, 90%; fetal bovine serum, 10%
|
| Subculturing |
Protocol: - Remove and discard culture medium.
-
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
-
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. -
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
-
Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37.0°C |
| Name of Depositor |
LW Anderson |
| Deposited As |
Mus musculus |
| References |
Anderson LW, et al. New cell line. Epithelial cell line and subline established from premalignant mouse mammary tissue. In Vitro 15: 841-843, 1979. PubMed: 232056
|
