| Applications |
This transformed cell line was derived from B-cells of a patient undergoing surgical resection of colorectal cancer. Peripheral blood mononuclear cells (PBMNC) were transformed with Epstein-Barr virus (EBV) derived from the B95-8 marmoset cell line. Co88BV59H21-2 cells produce monoclonal antibody 88BV59 that has been shown to recognize colon tumor associated antigen (CTAA) 16.88, also referred to as CTA #1. The CO 88BV59 cell line was fused with a human-mouse heteromyeloma to produce a cell line designated Co88BV59H21-2 (ATCC CRL-11538). Co88BV59H21-2 was then exposed to EBV under conditions suitable for transformation to produce Co88BV59H21-2V67-66 (ATCC CRL-11539). |
| Comments |
This transformed cell line was derived from B-cells of a patient undergoing surgical resection of colorectal cancer. The patient was actively immunized with autologous tumor antigen.
Peripheral blood mononuclear cells (PBMNC) were transformed with Epstein-Barr virus (EBV) derived from the B95-8 marmoset cell line.
Co88BV59H21-2 cells produce monoclonal antibody 88BV59 that has been shown to recognize colon tumor associated antigen (CTAA) 16.88, also referred to as CTA #1.
Both the 88BV59 antibody and the 16-88 antibody recognize the same tumor associated antigen, but react with different epitopes on that antigen.
The 16-88 antibody (LiCO 16-88) is available as ATCC HB-8495.
The CO 88BV59 cell line was fused with a human-mouse heteromyeloma to produce a cell line designated Co88BV59H21-2 (ATCC CRL-11538).
Co88BV59H21-2 was then exposed to EBV under conditions suitable for transformation to produce Co88BV59H21-2V67-66 (ATCC CRL-11539). |
| References |
Hanna M Jr., et al. Tumor associated monocoloal antibodies derived from human B-cell line. US Patent 4,997,762 dated Mar 5 1991
Hanna MG Jr., et al. Tumor associated monoclonal antibodies. US Patent 5,474,755 dated Dec 12 1995
Butler SM, et al. Tumor associated epitope. US Patent 5,951,985 dated Sep 14 1999
De Jager R, et al. Current status of cancer immunodetection with radiolabeled human monoclonal antibodies. Semin. Nucl. Med. 23: 165-179, 1993. PubMed: 8511602
Moffat FL Jr., et al. Preoperative scintigraphy and operative probe scintimetry of colorectal carcinoma using technetium-99m-88BV59. J. Nucl. Med. 36: 738-745, 1995. PubMed: 7738642
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
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