| 產品名稱 |
COLO 320HSR [COLO 320 HSR] |
| 商品貨號 |
B164285 |
| Organism |
Homo sapiens, human |
| Tissue |
colon |
| Product Format |
frozen |
| Morphology |
cells are rounded and refractile |
| Culture Properties |
loosely adherent, multicell aggregates |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Dukes' type C, colorectal adenocarcinoma |
| Age |
55 years |
| Gender |
female |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
marker chromosomes with homogeneously staining regions (HSR) were observed and the double minute (DM) chromosomes appeared in much lower frequency than the parental line COLO 320DM |
| Clinical Data |
55 years Caucasian female |
| Genes Expressed |
serotonin; norepinephrine; epinephrine; adrenocorticotropic hormone (ACTH); parathyroid hormone |
| Cellular Products |
serotonin; norepinephrine; epinephrine; adrenocorticotropic hormone (ACTH); parathyroid hormone |
| Tumorigenic |
Yes |
| Effects |
Yes, in nude mice |
| Comments |
The cells are weakly positive for keratins and vimentin. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
-
Shake flask. Remove culture medium to a centrifuge tube.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually with 5 to 10 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
- To remove trypsin-EDTA solution, transfer cell suspension to the centrifuge tube with the medium and cells from step #1 and spin at approximately 125 x g for 5 to10 minutes.
- Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.
- Place culture vessels in incubators at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 3 to 4 days |
| Cryopreservation |
Freeze medium: FreezeMedium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 11 D13S317: 11 D16S539: 11,12 D5S818: 12 D7S820: 9,12 THO1: 8,9 TPOX: 8,9 vWA: 15,18 |
| Isoenzymes |
ES-D, 1 G6PD, B PEP-D, 1 PGD, A PGM1, 1 PGM3, 2 |
| Name of Depositor |
GE Moore |
| Deposited As |
Homo sapiens |
| References |
Clevenstine EC, et al. Cell lines from human colon carcinoma with unusual cell products, double minutes, and homogeneously staining regions. Cancer Res. 39: 4914-4924, 1979. PubMed: 498117
Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874
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