| 產(chǎn)品名稱 |
D1 ORL UVA [D1] |
| 商品貨號(hào) |
B164310 |
| Organism |
Mus musculus, mouse |
| Tissue |
bone marrow |
| Product Format |
frozen |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain |
BALB/c |
| Applications |
This cell line is a suitable transfection host.
The cells may be systemically administered to treat osteoporosis, osteolysis, improve bone implant adherence, augment bone growth or bone repair, augment cartilage repair, and augment fat production. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
The D1 cell line was cloned from a multipotent mouse bone marrow stromal precursor. |
| Clinical Data |
The cells are "homing" cells, that is, cells injected systemically into the patient home to the bone marrow, unless specifically placed in another location. |
| Comments |
The cells can differentiate into osteocytes, chondrocytes and adipocytes in the presense of the appropriate stimulus and environment.
The cells are "homing" cells, that is, cells injected systemically into the patient home to the bone marrow, unless specifically placed in another location.
The cells may be transformed with recombinant DNA for the expression of both reporter genes, and biological factors, such as growth factors.
|
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
- Remove and discard culture medium.
-
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
-
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
-
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
-
Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor |
University of Virginia |
| Deposited As |
mouse |
| U.S. Patent Number |
|
| References |
Balian G, et al. Pluripotential bone marrow cell line and methods of using the same. US Patent 6,082,364 dated Jul 4 2000
|
