| 產品名稱 |
D283 Med |
| 商品貨號 |
B164316 |
| Organism |
Homo sapiens, human |
| Tissue |
brain/cerebellum; derived from metastatic site: peritoneum |
| Cell Type |
Medulloblastoma |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
suspension, multicell aggregates and some adherent cells |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
medulloblastoma |
| Age |
6 years juvenile |
| Gender |
male |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
The karyotype is 45, XY, -7, -8, -17, -20, der(20)t(1;20)(q12;q13), 8q+, 17p+ (range = 41 to 46). This is a hypodiploid cell line with a frequency of higher ploidies of 5.4%. Three marker chromosomes are present in all cells. They are: der(20)t(1;20)(q12;q13), 8q+ and 17p+. N7, N17 and N20 have single copies. The single X is structurally normal, and the Y chromosome is present as confirmed by fluorescence microscopy. |
| Derivation |
The D283 Med cell line was established in 1985 by Friedman et al. from malignant ascites cells and a peritoneal metastasis from a boy with medulloblastoma. - Derived from metastatic site, peritoneum |
| Clinical Data |
6 years juvenile Caucasian The D283 Med cell line was established in 1985 by Friedman et al. from malignant ascites cells and a peritoneal metastasis from a boy with medulloblastoma. male |
| Genes Expressed |
The cells produce tumors in nude mice, and the resulting tumors are positive for expression of neurofibrillary proteins, glutamine synthetase and neuron specific enolase but negative for glial fibrillary acidic proteins and S100 (S-100) protein. The cells express elevated levels of four biochemical markers of SCLC (neuron specific enolase, the brain isoenzyme of creatine kinase, L-DOPA decarboxylase and bombesin-like immunoreactivity. |
| Cellular Products |
neurofilament proteins (NFP) |
| Tumorigenic |
Yes |
| Effects |
Yes, in nude mice (The cells form serially transplantable intercranial and subcutaneous tumors.) Yes, in soft agar |
| Comments |
The cells produce tumors in nude mice, and the resulting tumors are positive for expression of neurofibrillary proteins, glutamine synthetase and neuron specific enolase but negative for glial fibrillary acidic proteins and S100 (S-100) protein. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Cultures can be maintained by addition or replacement of medium. Adherent cells can be dislodged by scraping, and cultures can be established by resuspending a cell pellet at 5 X 105viable cells/mL. Maintain cultures between 4 X 105 and 1 X 105 cells/mL.
Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
Freeze medium: culture medium, 90%; DMSO, 10% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile |
Amelogenin: X,Y CSF1PO: 9,12 D13S317: 8,10 D16S539: 11 D5S818: 11 D7S820: 10 THO1: 7 TPOX: 8,11 vWA: 16,18 |
| Isoenzymes |
AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 0 PGM1, 1 PGM3, 1 |
| Population Doubling Time |
52 hrs |
| Name of Depositor |
HS Friedman |
| Deposited As |
Homo sapiens |
| Year of Origin |
1985 |
| References |
Friedman HS, et al. Phenotypic and genotypic analysis of a human meduloblastoma cell line and transplantable xenograft (D341 Med) demonstrating amplification of c-myc. Am. J. Pathol. 130: 472-484, 1988. PubMed: 3279793
He XM, et al. Expression of O6-methylguanine-DNA methyltransferase in six human medulloblastoma cell lines. Cancer Res. 52: 1144-1148, 1992. PubMed: 1737373
Friedman HS, et al. Establishment and characterization of the human medulloblastoma cell line and transplantable xenograft D283 Med. J. Neuropathol. Exp. Neurol. 44: 592-605, 1985. PubMed: 4056828
Trojanowski JQ, et al. A rapidly dividing human medulloblastoma cell line (D283 MED) expresses all three neurofilament subunits. Am. J. Pathol. 126: 358-363, 1987. PubMed: 3103453
Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024
The cells form serially transplantable intercranial and subcutaneous tumors.
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