| Applications |
The hybridoma cell line DAL K45 secretes a mouse monoclonal antibody (IgG1) reactive with human renal cell carcinoma; it does not react significantly with normal tissues. This antibody as well as DAL K20 (ATCC CRL-2288) and DAL 29 (ATCC CRL-2291) is useful for identifying kidney differentiation antigens and has been shown effective in radioimaging as well as tumor growth inhibition in xenograft models. The line was produced by fusing SP2 myeloma cells with spleen cells from BALB/c mice that had been immunized with the Caki-1 clear cell kidney carcinoma cell line. |
| Comments |
The hybridoma cell line DAL K45 secretes a mouse monoclonal antibody (IgG1) reactive with human renal cell carcinoma; it does not react significantly with normal tissues.
DAL K45 precipitates molecules with molecular weighs of 177,000 and 150,000 from extracts of surface-labeled Caki-1 (ATCC HTB-46) cells.
This antibody as well as DAL K20 (ATCC CRL-2288) and DAL 29 (ATCC CRL-2291) is useful for identifying kidney differentiation antigens and has been shown effective in radioimaging as well as tumor growth inhibition in xenograft models.
The line was produced by fusing SP2 myeloma cells with spleen cells from BALB/c mice that had been immunized with the Caki-1 clear cell kidney carcinoma cell line. |
| References |
Luner SJ, et al. Monoclonal antibodies to kidney and tumor-associated surface antigens of human renal cell carcinoma. Cancer Res. 46: 5816-5820, 1986. PubMed: 3530441
Guha AK, et al. Tumor localization of monoclonal antibodies against human renal carcinoma in a xenograft model. Cancer Lett. 61: 35-43, 1991. PubMed: 1764696
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
|
