| 產品名稱 |
DBTRG-05MG |
| 商品貨號 |
B164338 |
| Organism |
Homo sapiens, human |
| Tissue |
brain |
| Cell Type |
glial cell |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
glioblastoma |
| Age |
59 years |
| Gender |
female |
| Ethnicity |
Caucasian |
| Karyotype |
near tetraploid; range 87 to 91; most cells were missing copies of chromosome 10 and had extra copies of chromosome 7 |
| Derivation |
The DBTRG-O5MG (Denver Brain Tumor Research Group 05) cell line was established from tissue from a patient with glioblastoma multiforme who had been treated with local brain irradiation and multidrug chemotherapy. |
| Clinical Data |
59 years
Caucasian
female
|
| Antigen Expression |
Class I antigen expressed |
| Receptor Expression |
epidermal growth factor (EGF) |
| Genes Expressed |
vimentin; S-100 protein; neuron specific enolase |
| Cellular Products |
vimentin; S-100 protein; neuron specific enolase |
| Comments |
The cells are negative for platelet derived growth factor (PDGF), neuronal cell adhesion molecule (NCAM), glial fibrillary acid protein (GFAP) and class II antigen (HLA DR).
No loss of heterozygosity in the p53 tumor suppressor gene was detected. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.
To make the complete growth medium, add the following components to the base medium:
- fetal bovine serum to a final concentration of 10%
- additional 30 mg/L L-proline
- additional 35 mg/L L-cystine
- additional 3.57 g/L HEPES
- 15 mg/L hypoxanthine
- 1 mg/L adenosine triphosphate
- 10 mg/L adenine
- 1 mg/L thymidine
|
| Subculturing |
Remove medium, add fresh 0.25% trypsin, rinse and remove trypsin. Let the flask sit at room temperature (or incubate at 37°C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended Medium Renewal: Every 2 to 3 days |
| STR Profile |
Amelogenin: X CSF1PO: 10,11 D13S317: 9 D16S539: 10,12 D5S818: 12,13 D7S820: 11 THO1: 7,8 TPOX: 8 vWA: 15,16 |
| Name of Depositor |
CA Kruse |
| Deposited As |
Homo sapiens |
| References |
Kruse CA, et al. Characterization of a continuous human glioma cell line DBTRG-05MG: growth kinetics, karyotype, receptor expression, and tumor suppressor gene analyses. In Vitro Cell. Dev. Biol. 28A: 609-614, 1992. PubMed: 1331021
Koochekpour S, et al. Met and hepatocyte growth factor/scatter factor expression in human gliomas. Cancer Res. 57: 5391-5398, 1997. PubMed: 9393765
Debinski W, et al. Receptor for interleukin (IL) 13 does not interact with IL4 but receptor for IL4 interacts with IL13 on human glioma cells. J. Biol. Chem. 271: 22428-22433, 1996. PubMed: 8798406
|
