| Subculturing |
To dislodge adherent cells, remove all but 5 to 10 mL of medium and vigorously shake flask. Add fresh medium, aspirate and dispense into new flasks. Subcultures may also be prepared using standard trypsinization procedures.
Subcultivation Ratio: A subcultivation ratio of 1:20 is recommended
Medium Renewal: 2 to 3 times per week |
| References |
Syms AJ, et al. Proliferation of a highly androgen-sensitive ductus deferens cell line (DDT MF-2) is regulated by glucocorticoids and modulated by growth collagen. In Vitro 19: 929-936, 1983. PubMed: 6662552
Smith RG, et al. Differential effects of androgens and glucocorticoids on regulation of androgen receptor concentrations and cell growth. J. Steroid Biochem. 20: 277-281, 1984. PubMed: 6708513
Norris JS, et al. Autocrine regulation of growth: II. Glucocorticoids inhibit transcription of c-sis oncogene-specific RNA transcripts. Biochem. Biophys. Res. Commun. 122: 124-128, 1984. PubMed: 6743325
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
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