| Comments |
Growth of DDT1-MF-2 cells is stimulated by androgens and inhibited by glucocorticoids. Glucocorticoids inhibit the expression of the c-sis (SIS) proto-oncogene. Beta-2-adrenergic (beta 2 adrenergic) receptor activity is functionally coupled to adenyl cyclase. This a deposit of DDT1-MF-2 (see ATCC CRL-1701) for patent purposes. |
| Subculturing |
To dislodge adherent cells, remove all but 5 to 10 mL of medium and vigorously shake flask. Add fresh medium, aspirate and dispense into new flasks. Subcultures may also be prepared using standard trypsinization procedures.
Subcultivation Ratio: A subcultivation ratio of 1:20 is recommended
Medium Renewal: 2 to 3 times per week |
| References |
Smith RG, et al. Differential effects of androgens and glucocorticoids on regulation of androgen receptor concentrations and cell growth. J. Steroid Biochem. 20: 277-281, 1984. PubMed: 6708513
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
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