| 產(chǎn)品名稱 | DH82 |
|---|---|
| 商品貨號 | B164356 |
| Organism | Canis familiaris, dog |
| Cell Type | Macrophage-like |
| Product Format | frozen |
| Morphology | macrophage |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | malignant histiocytosis |
| Age | 10 years |
| Gender | male |
| Storage Conditions | liquid nitrogen vapor phase |
| Disclosure | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Images |  |
| Clinical Data | dog; golden retriever
male
10 years |
| Comments | The DH82 cell line has a macrophage-like morphology, and is able to phagocytize latex particles. They are positive for Fc gamma receptors and negative for Fc mu and C3b receptors. The cells do not produce IL-1. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 15%.
|
| Subculturing | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation | culture medium 95%; DMSO, 5% |
| Culture Conditions | Temperature: 37°C |
| Name of Depositor | The United States of America |
| U.S. Patent Number | |
| References | Dawson JE, Rikihisa Y. Growing Ehrlichia species in a continuous cell line. US Patent 5,192,679 dated Mar 9 1993 Wellman ML, et al. A macrophage-monocyte cell line from a dog with malignant histiocytosis. In Vitro Cell. Dev. Biol. 24: 223-229, 1988. PubMed: 3350786 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
