| 產(chǎn)品名稱 |
DSN |
| 商品貨號(hào) |
B164375 |
| Organism |
Canis familiaris, dog |
| Tissue |
bone; Derived from metastatic site: lung |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
2 Cells contain CMV, SNV, SV-40 viral DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
osteosarcoma |
| Age |
11 years |
| Gender |
female |
| Strain |
poodle |
| Applications |
The DSN cell line was derived from D-17 (see ATCC CCL-183) cells by transfecting with plasmids containing the gag-pol and the env genes of spleen necrosis virus. The line is for use as a helper cell for propagating replication incompetent vectors derived from the SNV retrovirus. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
The DSN cell line was derived from D-17 (see ATCC CCL-183) cells by transfecting with plasmids containing the gag-pol and the env genes of spleen necrosis virus. The line is for use as a helper cell for propagating replication incompetent vectors derived from the SNV retrovirus. |
| Clinical Data |
female |
| Comments |
The DSN cell line was derived from D-17 (see ATCC CCL-183) cells by transfecting with plasmids containing the gag-pol and the env genes of spleen necrosis virus. The line is for use as a helper cell for propagating replication incompetent vectors derived from the SNV retrovirus. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:12 is recommended Medium Renewal: Every 2 to 3 days Remove medium, add fresh 0.25% trypsin-0.02% EDTA for several minutes, remove trypsin and let the culture sit at 37C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 4 to 6 days. |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Name of Depositor |
Wisconsin Alumni Res. Fndn. |
| Deposited As |
Canis familiaris |
| U.S. Patent Number |
|
| References |
Temin HM, Dougherty JP. Recombination resistant retroviral helper cell and products produced thereby. US Patent 5,124,263 dated Jun 23 1992
|
