| 產品名稱 |
Duck embryo |
| 商品貨號 |
B164382 |
| Organism |
Anas platyrhynchus domesticus, duck, Pekin |
| Tissue |
embryo |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age |
embryo |
| Storage Conditions |
liquid nitrogen vapor phase |
| Virus Susceptibility |
Vesicular stomatitis, Orsay (Indiana)
Vesicular stomatitis, Glasgow (Indiana)
Herpes simplex virus
St. Louis encephalitis virus
,
St. Louis encephalitis virus
California encephalitis virus
,
Melao virus
Eastern equine encephalitis virus
,
Eastern equine encephalitis virus
Anatid herpesvirus 1
Rubella virus
,
Rubella virus
|
| Virus Resistance |
poliovirus 2 |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Volumes used in this protocol are for a 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. It is important to subculture the cells as soon as they become confluent, or else the cells will begin to die.
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum, which contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. Do not attempt to break up cell clumps.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: 1:2 to 1:6.
Medium Renewal: 1 to 2 times per week |
| Cryopreservation |
Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor |
M Marcovici, J Prier, M Allen |
| Deposited As |
Anas platyrhynchus domesticus |
| References |
Marcovici M, Prier JE. Enhancement of St. Louis arbovirus plaque formation by neutral red. J. Virol. 2: 178-181, 1968. PubMed: 4911850
Melnick JL. Tissue culture techniques and their application to original isolation, growth, and assay of poliomyelitis and orphan viruses. Ann. N.Y. Acad. Sci. 61: 754-772, 1955. PubMed: 13340582
Wolf K, et al. Duck viral enteritis: microtiter plate isolation and neutralization test using the duck embryo fibroblast cell line. Avian Dis. 18: 427-434, 1974. PubMed: 4368600
Buynak EB, et al. Preparation and testing of duck embryo cell culture rubella vaccine. Am. J. Dis. Child. 118: 347-354, 1969. PubMed: 4307520
Farris AD, et al. Conserved features of Y RNAs revealed by automated phylogenetic secondary structure analysis. Nucleic Acids Res. 27: 1070-1078, 1999. PubMed: 9927741
|
| Cross References |
Nucleotide (GenBank) :
U82124
Anas platyrhynchus Y1 RNA, partial sequence.
Nucleotide (GenBank) :
U82125
Anas platyrhynchus Y3 RNA, partial sequence.
|
