Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
Tested and found negative for ectromelia virus (mousepox).
Virus Resistance
poliovirus 3
Comments
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
NCTC 135 medium, 90%; fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Subcultures can be prepared by scraping. Remove old medium, add fresh, dislodge cells from the floor of the flask, aspirate and dispense into new flasks. Alternatively, a standard trypsinization procedure can be used.
Name of Depositor
CW Boone
Deposited As
Mus musculus
References
Boone C, et al. Characterization of an in vitro strain of Ehrlich-Lettre ascites carcinoma subjected to many periodic mouse passages. J. Natl. Cancer Inst. 34: 725-740, 1965. PubMed: 5829538
