| 產品名稱 |
EIAV 12E8.1 |
| 商品貨號 |
B164399 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against equine infectious anemia virus (EIA, EIAV) core antigen (p26) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against equine infectious anemia virus (EIA, EIAV) core antigen (p26) |
| Comments |
Animals were immunized with p26 prepared from the Wyoming strain of EIA virus. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Complete Growth Medium |
RPMI 1640 medium with 2 mM L-glutamine, 85%; fetal bovine serum, 15%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
DMEM, 70%; fetal bovine serum, 20%; DMSO, 10% |
| Isotype |
IgG1 |
| Name of Depositor |
Fermenta Animal Health Company |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Matsushita T, et al. Method for the detection of equine infectious anemia and other retrovirus infections using a competitive enzyme-linked immunoabsorbent assay and reagents useful in the same. US Patent 4,806,467 dated Feb 21 1989
|
