| 產品名稱 |
EL-NC-1S [anti NAP-1] |
| 商品貨號 |
B164408 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain |
Strain: BALB/c (B cell); BALB/c (myeloma) |
| Applications |
Animals were immunized with purified monocyte derived neutrophil chemotactic factor (MDNCF). |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Animals were immunized with purified monocyte derived neutrophil chemotactic factor (MDNCF). Spleen cells were fused with NS-1 mouse myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against neutrophil attractant/activation protein 1 (NAP-1, also known as interleukin 8 or IL-8 and monocyte derived neutrophil chemotactic factor, MDNCF) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against neutrophil attractant/activation protein 1 (NAP-1, also known as interleukin 8 or IL-8 and monocyte derived neutrophil chemotactic factor, MDNCF) |
| Comments |
Animals were immunized with purified monocyte derived neutrophil chemotactic factor (MDNCF). Spleen cells were fused with NS-1 mouse myeloma cells. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG1 |
| Name of Depositor |
National Cancer Institute/FCRF |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Yoshimura T, et al. Three forms of monocyte-derived neutrophil chemotactic factor (MDNCF) distinguished by different lengths of the amino-terminal sequence. Mol. Immunol. 26: 87-93, 1989. PubMed: 2648135
Sylvester I, et al. Secretion of neutrophil attractant/activation protein by lipopolysaccharide-stimulated lung macrophages determined by both enzyme-linked immunosorbent assay and N-terminal sequence analysis. Am. Rev. Respir. Dis. 141: 683-688, 1990. PubMed: 2178529
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