| 產品名稱 | EOMA |
|---|---|
| 商品貨號 | B164413 |
| Organism | Mus musculus, mouse |
| Tissue | tumor |
| Cell Type | endothelial |
| Product Format | frozen |
| Morphology | endothelial |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | hemangioendothelioma |
| Age | adult |
| Strain | 129 |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | The EOMA cell line was originally derived in 1980 from a mixed hemangioendothelioma arising in an adult mouse. |
| Antigen Expression | CD31 + vascular addressin + CD45 (Ly5-T200) + |
| Receptor Expression | acetylated low density liproprotein Ref   Obeso J, et al. A hemangioendothelioma-derived cell line: its use as a model for the study of endothelial cell biology. Lab. Invest. 63: 259-269, 1990. PubMed: 2166185 |
| Genes Expressed | angiotensin converting enzyme (ACE)
thrombospondin
cathepsin L
endostatin
interleukin-6 (interleukin 6, IL-6)
|
| Cellular Products | angiotensin converting enzyme (ACE)
thrombospondin
cathepsin L
endostatin
interleukin-6 (interleukin 6, IL-6) |
| Tumorigenic | Yes |
| Effects | Yes, in syngeneic mice |
| Comments | The cells synthesize angiotensin-converting enzyme, express surface receptors for acetylated low density lipoprotein, produce thrombospondin and show intracellular staining with an antibody to von Willebrand factor. Cathepsin L is secreted by EOMA cells and is responsible for the generation of endostatin L. Although constitutive cytokine gene expression exists in EOMA cells, the level of IL-6 mRNA is prominently elevated by incubation with Liposome encapsulated hemoglobin (LEH). The cells constitutively express the vascular addressin identified by antibody MECA-99. EOMA cells exhibit characteristic endothelial cell properties, such as rearrangement into tubelike structures on Matrigel and retention of cobblestone morphology at confluence. They behave in vitro in a manner similar to microvascular endothelial cells. |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture Ratio: 1:3 to 1:6 Medium Renewal: Every 2 to 3 days.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994 |
| Cryopreservation | Freeze medium: Complete growth medium supplemented with 5% DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Temperature: 37°C |
| Name of Depositor | R Auerbach |
| Deposited As | mouse |
| Year of Origin | 1980 |
| References | Felbor U, et al. Secreted cathepsin L generates endostatin from collagen XVIII. EMBO J. 19: 1187-1194, 2000. PubMed: 10716919 Obeso J, et al. A hemangioendothelioma-derived cell line: its use as a model for the study of endothelial cell biology. Lab. Invest. 63: 259-269, 1990. PubMed: 2166185 Zhu XL, et al. Kinetics of cytokine gene expression in macrophage and endothelial cell lines following liposome encapsulated haemoglobin (LEH) treatment in vitro. Cytokine 8: 541-547, 1996. PubMed: 8891435 Wen W, et al. The generation of endostatin is mediated by elastase.. Cancer Res. 59: 6052-6056, 1999. PubMed: 10626789 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
