| 產品名稱 |
FW11-10-3 |
| 商品貨號 |
B164472 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Animals were immunized with a fusion protein corresponding to the variant region of human CD44 (exons 3v to 10v). The antibody is specific for the product of the 4v exon. |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against the v4 variant of human CD44 |
| Cellular Products |
immunoglobulin; monoclonal antibody; against the v4 variant of human CD44 |
| Comments |
Animals were immunized with a fusion protein corresponding to the variant region of human CD44 (exons 3v to 10v). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody is specific for the product of the 4v exon. |
| Complete Growth Medium |
Culture Medium: Iscove's modified Dulbecco's medium, 90%; fetal bovine serum,
10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Isotype |
IgG2a |
| Name of Depositor |
CR Mackay |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Mackay CR, et al. Expression and modulation of CD44 variant isoforms in humans. J. Cell Biol. 124: 71-82, 1994. PubMed: 7507492
|
