| 產品名稱 |
G2a.5 |
| 商品貨號 |
B164479 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against HLA DC1 (HLA DR) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against HLA DC1 (HLA DR) |
| Comments |
Animals were immunized with glycoprotein fraction from the Bristol 8 human B lymphoblastoid cell line. Spleen cells were fused with NS-1 myeloma cells. This line is a switch variant of Genox 3.53 (ATCC HB-103). An IgG2b switch variant of Genox 3.53 also exists (G2b.2, ATCC HB-109). Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Culture Medium: RPMI medium 1640 with HT (containing: hypoxanthine, 10(-4) M;
thymidine, 1.6 x 10(-5) M), 90%; fetal bovine serum, 10%.
Cells do not do well if hypoxanthine and thymidine are omitted. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG2a |
| Name of Depositor |
P Parham |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Parham P, et al. Isolation of heavy chain class switch variants of a monoclonal anti-DC1 hybridoma cell line: effective conversion of noncytotoxic IgG1 antibodies to antibodies. Hum. Immunol. 8: 141-151, 1983. PubMed: 6417068
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