| Applications |
They are slightly responsive to acetylcholine. To avoid this, the cells should be subcultured before they become confluent, and they should be recloned periodically with selection for myoblastic clones. |
| Comments |
When confluent, the cells fuse to form multinucleated myotubes. They are slightly responsive to acetylcholine. The myoblastic component of the population will rapidly become depleted if the cells are allowed to become confluent, since more extensive fusion will take place. To avoid this, the cells should be subcultured before they become confluent, and they should be recloned periodically with selection for myoblastic clones. |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 80%; horse serum, 10%; fetal bovine serum, 10%
Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 80%; horse serum, 10%; fetal bovine serum, 10%
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| References |
Christian CN, et al. Synapse formation between two clonal cell lines. Science 196: 995-998, 1977. PubMed: 193191
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
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