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GA-10 (Clone 20)

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編號(hào):B164488

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產(chǎn)品名稱	GA-10 (Clone 20)
商品貨號(hào)	B164488
Organism	Homo sapiens, human
Cell Type	B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	Burkitt's lymphoma
Age	41 years
Gender	male
Ethnicity	Caucasian, White
Storage Conditions	liquid nitrogen vapor phase
Derivation	GA-10 (Clone 20) is a subclone of the GA-10 (ATCC CRL-2392) cell line derived by culturing the parental line on semi-solid medium conditioned by a feeder layer. GA-10 was derived in 1996 from lymphoma cells circulating in the peripheral blood of a patient with a clinically aggressive, chemo-refractory Burkitt's Lymphoma.
Clinical Data	The patient had been treated unsuccessfully with cyclophosphamide, hydroxydaunomycin, vincristine, and prednisone (CHOP chemotherapy). male Caucasian, White 41 years
Antigen Expression	CD77 + (80-90%)
Comments	This clone of cells also expresses high levels of CD77 (80-90%); CD77 is also known as Burkitt's lymphoma antigen (BLA). GA-10 (Clone-20) cells exhibit marked sensitivity to Shiga Toxin (Stx) in cytotoxicity studies.
Complete Growth Medium	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing	Cultures can be maintained by addition of fresh Medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 x 10⁵ viable cells/mL. Maintain cultures at a cell concentration between 5 x 10⁵ and 2 x 10⁶ cells/mL. Do not allow the cell concentration to exceed 2 x 10⁶ cells/mL. Medium Renewal: Every 2 to 3 days
Cryopreservation	Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
STR Profile	Amelogenin: X,Y CSF1PO: 10 D13S317: 11 D16S539: 11,12 D5S818: 11 D7S820: 8,10 THO1: 9,9.3 TPOX: 8,11 vWA: 16,20
Name of Depositor	AP Rapoport
Deposited As	human
References	Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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