| 產品名稱 |
GAP A3 |
| 商品貨號 |
B164494 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension, suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against HLA A3 |
| Cellular Products |
immunoglobulin; monoclonal antibody; against HLA A3 |
| Comments |
Animals were immunized with human peripheral blood lymphocytes. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody does not react with HLA A11. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell culture grade water. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10% 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
|
| Subculturing |
Protocol: Cultures can be maintained by addition or replacement of medium. Establish new cultures at 1 X 10(5) viable cells/ml and maintain between 2 X 10(5) and 1 X 10(6) cells/ml. Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Isotype |
IgG2a; kappa light chain |
| Name of Depositor |
AE Berger |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
. . Fed. Proc. 42: 1371, 1983.
Dieckmann CL, et al. Monoclonal antibody to HLA-A3. Hybridoma 1: 87-90, 1982. PubMed: 6208124
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