| 產品名稱 |
GH3 |
| 商品貨號 |
B164503 |
| Organism |
Rattus norvegicus, rat |
| Tissue |
pituitary |
| Cell Type |
Epithelial-like |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
loosely adherent with floating clusters |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
tumor |
| Age |
7 months |
| Gender |
female |
| Strain |
Wistar-Furth |
| Applications |
This cell line is a suitable transfection host. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
modal number = 67; range = 47 to 71.
Stemline karyotype is stable with a few structural alterations. Two dicentric marker chromosomes were observed in 100% of the cells examined. |
| Derivation |
Although the GH3 was not a direct derivative of the GH1 clone (ATCC CCL-82), it was initiated after only two additional animal passages from the primary culture from which the GH1 clone was established.
Clone GH3 was established in July, 1965 by A.H. Tashjian, Jr., et al. from a pituitary tumor carried in a 7-month-old female Wistar-Furth rat. |
| Clinical Data |
female
7 months |
| Genes Expressed |
prolactin; growth hormone (somatotrophin) |
| Cellular Products |
prolactin; growth hormone (somatotrophin) |
| Virus Susceptibility |
Herpes simplex virus
Vesicular stomatitis virus
Human poliovirus 1
|
| Comments |
The epithelial-like GH3 clone generates growth hormone at a greater rate than the GH1 cells and also produces prolactin.
Studies on the control of the production of these protein hormones by the GH3 cells have shown that hydrocortisone stimulates the production of growth hormone and inhibits prolactin production.
The cells have been adapted to growth in suspension culture using Eagle's minimum essential medium (spinner) supplemented with 15% horse serum and 2.5% FBS. Under these conditions the cells continue to produce both growth hormone and prolactin |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5%; horse serum to a final concentration of 15%.
|
| Subculturing |
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
- Remove and discard culture medium. Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it.
-
Add 2.0 to 3.0 mL of 0.25% (w/v) Trypsin- 0.53 mM-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
-
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
-
Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| Name of Depositor |
AH Tashjian |
| Deposited As |
Rattus sp. |
| Passage History |
Although the GH3 was not a direct derivative of the GH1 clone (ATCC CCL-82), it was initiated after only two additional animal passages from the primary culture from which the GH1 clone was established.
The cell line was submitted to the American Type Culture Collection in October, 1972 in the 10th subculture generation.
|
| Year of Origin |
July, 1965 |
| References |
Bancroft FC, et al. Control of growth hormone production by a clonal strain of rat pituitary cells. Stimulation by hydrocortisone. J. Cell Biol. 43: 432-441, 1969. PubMed: 5389137
Tashjian AH Jr., et al. Establishment of clonal strains of rat pituitary tumor cells that secrete growth hormone. Endocrinology 82: 342-352, 1968. PubMed: 4951281
Tashjian AH Jr., et al. Production of both prolactin and growth hormone by clonal strains of rat pituitary tumor cells. J. Cell Biol. 47: 61-70, 1970. PubMed: 5513559
Bancroft FC, Tashjian AH Jr.. Growth in suspension culture of rat pituitary cells which produce growth hormone and prolactin. Exp. Cell Res. 64: 125-128, 1971. PubMed: 5541958
Bancroft FC, Tashjian AH Jr.. Control of the production of two protein hormones by rat pituitary cells in culture. In Vitro 6: 180-189, 1970. PubMed: 5535574
Wood WM, et al. Thyroid hormone receptor beta2 promoter activity in pituitary cells is regulated by Pit-1. J. Biol. Chem. 271: 24213-24220, 1996. PubMed: 8798664
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