波多野结衣毛片-乳色吐息在线观看-国产伦子伦对白视频-性做久久久-狠狠干2019-黄色裸体片-美女无遮挡免费网站-国产91熟女高潮一区二区-懂色av蜜臀av粉嫩av分享-小h片在线观看-台湾佬在线-日韩激情在线播放-欧日韩不卡在线视频-波多野结衣中文字幕一区-天天操夜夜草

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > GK1.5
最近瀏覽歷史
聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
GK1.5
GK1.5
規(guī)格:
貨期:
編號:B164506
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 GK1.5
商品貨號 B164506
Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Tissue Spleen
Cell Type Hybridoma: B lymphocyte
Product Format frozen
Morphology Lymphoblast-like
Culture Properties Suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain Lewis (B cell); BALB/c (myeloma)
Applications
The antibody profoundly blocks antigen specific murine class II MHC antigen reactive helper T lymphocyte lines.
Storage Conditions liquid nitrogen vapor phase
Derivation

Animals were immunized with the cloned cytotoxic T lymphocyte lines V4 and 243/2.5.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

Genes Expressed
immunoglobulin; monoclonal antibody; against mouse helper, inducer T cells (L3T4 antigen, CD4)
Cellular Products immunoglobulin; monoclonal antibody; against mouse helper, inducer T cells (L3T4 antigen, CD4)
Comments
L3T4 is expressed on mouse helper/inducer T cells and is analogous to the human Leu-3/T4 molecule.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10viable cells/mL. Maintain cultures at a cell concentration between 1 x 105 and 1 x 10cells/mL. Do not allow the cell concentration to exceed 1 x 10cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Complete growth medium supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype Rat IgG2b
Name of Depositor FW Fitch
Deposited As rat (B cell); mouse (myeloma)
References

Fitch FW, et al. Evidence implicating L3T4 class II MHC antigen reactivity; monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen-specific proliferation, release of lymphokines, and binding by cloned murine helper T lymphocyte lines. J. Immunol. 131: 2178-2183, 1983. PubMed: 6195255

Dialynas DP, et al. Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule. J. Immunol. 131: 2445-2451, 1983. PubMed: 6415170

Garvy BA, Harmsen AG. The role of T cells in infection-driven interstitial pneumonia after bone marrow transplantation in mice. Transplantation 62: 517-525, 1996. PubMed: 8781619

Murray HW, et al. Models of relapse of experimental visceral leishmaniasis. J. Infect. Dis. 173: 1041-1043, 1996. PubMed: 8603949

Wilson ME, et al. Local suppression of IFN-gamma in hepatic granulomas correlates with tissue-specific replication of Leishmania chagasi. J. Immunol. 156: 2231-2239, 1996. PubMed: 8690913

Wong P, Rudensky AY. Phenotype and function of CD4+ T cells in mice lacking invariant chain. J. Immunol. 156: 2133-2142, 1996. PubMed: 8690902

Sayles PC, Johnson LL. Intact immune defenses are required for mice to resist the ts-4 vaccine strain of Toxoplasma gondii. Infect. Immun. 64: 3088-3092, 1996. PubMed: 8757838

Murray HW, et al. Multiple host defense defects in failure of C57BL/6 ep/ep (Pale Ear) mice to resolve visceral Leishmania donovani infection. Infect. Immun. 64: 161-166, 1996. PubMed: 8557335

Dialynas DP, et al. Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with class II MHC antigen-reactivity. Immunol. Rev. 74: 29-56, 1983. PubMed: 6195085

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo JL. Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
主站蜘蛛池模板: 高清在线一区二区三区 | 精品国产亚洲av麻豆 | 蜜臀av性久久久久蜜臀av麻豆 | 丁香激情五月 | 一区二区高清在线 | 欧美在线三级 | 亚洲免费在线视频观看 | 极品三级 | 国产精品久久久久久久久夜色 | 国产精品久久久久久久久久妞妞 | 樱桃国产成人精品视频 | 国产毛片久久 | 鲁大师私人影院在线观看 | 神马影院午夜伦理 | 亚洲女人天堂av | 无遮挡裸光屁屁打屁股男男 | 青青草原综合久久大伊人精品 | 免费男女乱淫真视频免费播放 | 黄色片免费网站 | 国产又黄又粗又猛又爽 | 亚洲一区中文字幕在线观看 | 男女叼嘿视频 | 91九色精品| 亚洲天堂男人的天堂 | 在线观看欧美一区 | 精品国自产在线观看 | 国内一级黄色片 | 97香蕉久久夜色精品国产 | 三上悠亚一区二区三区 | 午夜色图 | 久久久噜噜噜久久久 | 国产一区二区精品在线观看 | 色综合五月 | 欧美日韩二区三区 | 久久国产99 | 亚洲成人一区在线观看 | 手机在线免费观看av | 久久久美女 | 国产午夜亚洲精品午夜鲁丝片 | 超碰丝袜| 新婚夫妇白天啪啪自拍 | 亚洲精品tv| 色香色香欲天天天影视综合网 | 538国产精品视频一区二区 | 日韩欧美一二区 | 操天天| 日韩不卡中文字幕 | 成人午夜福利视频 | 日韩二级| 91精品人妻一区二区三区蜜桃2 | 亚洲国产视频网站 | 国产亚洲成人av | 国产网站精品 | 亚洲色图欧美在线 | 在线国产日韩 | 2023天天操| 国产精品福利一区 | 国产福利久久久 | 毛片手机在线 | 国产成人a亚洲精品 | 国产成人无码一区二区在线播放 | 成年人一级黄色片 | 色淫湿视频 | 人人上人人干 | www.夜色 | 蜜臀久久精品久久久久 | 亚洲作爱视频 | 国产成人精品一区二三区 | 一级视频在线观看 | 精品亚洲一区二区三区 | 久久人人妻人人人人妻性色av | 波多野在线视频 | 欧美一级淫片免费视频魅影视频 | 日韩一卡二卡 | 秋霞在线视频观看 | 亚洲成人av免费观看 | 日韩欧美成人一区二区三区 | 欧洲精品久久一区二区 | 国产黄色在线网站 | 中文字幕一二三四 | 日韩经典一区二区三区 | 欧美日韩爱爱 | 欧美一区亚洲二区 | 超碰男人的天堂 | www在线观看免费视频 | av东方在线 | 色视频一区 | 久久系列| 麻豆国产91在线播放 | 国产suv精品一区二区69 | 男人天堂视频在线观看 | 亚洲熟妇av乱码在线观看 | 95在线视频| 久久久久久无码精品大片 | 95视频在线 | 内射干少妇亚洲69xxx | 国产白丝一区二区三区 | 久久久久中文字幕亚洲精品 | 国产靠逼视频 |