| 產(chǎn)品名稱 |
GP+envAM-12 |
| 商品貨號 |
B164513 |
| Organism |
Mus musculus, mouse |
| Tissue |
embryo |
| Cell Type |
fibroblast |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age |
embryo |
| Strain |
NIH/Swiss |
| Applications |
It can be used to produce retroviral vectors for delivery of foreign genes into susceptible eukaryotic cells.
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
The line was established by electroporation into NIH 3T3 cells of two plasmids that separately encode the the env region of a murine amphotropic MuLV and the gag, pol and other sequences needed for viral packaging. |
| Comments |
This line is capable of packaging nucleic acids containing a psi packaging sequence into recombinant amphotropic retrovirus genomes.
|
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002.
To make the complete growth medium, add the following components to the base medium:
• bovine calf serum to a final concentration of 10%
After 1 to 3 days, add
• 0.015 mg/ml hypoxanthine
• 0.25 mg/ml xanthine
• 0.025 mg/ml mycophenolic acid
• 0.2 mg/ml hygromycin B |
| Subculturing |
Remove medium, add fresh 0.10% trypsin in phosphate buffered saline for 3 to 5 minutes, remove trypsin and let the culture sit at 37°C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks. Medium Renewal: Twice per week |
| Culture Conditions |
Temperature: 37°C |
| Name of Depositor |
Trustees of Columbia University |
| Deposited As |
Mus musculus |
| U.S. Patent Number |
|
| References |
Bank A, et al. Retroviral packaging cell lines and process of using same. US Patent 5,278,056 dated Jan 11 1994
Medin JA, et al. Correction in trans for Fabry disease: expression, secretion, and uptake of alpha-galactosidase A in patient-derived cells driven by a high-titer recombinant retroviral vector. Proc. Natl. Acad. Sci. USA 93: 7917-7922, 1996. PubMed: 8755577
|
