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H16-L10-4R5

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編號:B164523

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產品名稱	H16-L10-4R5
商品貨號	B164523
Organism	Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type	hybridoma: B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Storage Conditions	liquid nitrogen vapor phase
Derivation	Mediastinal lymphocytes from BALB/c mice infected with influenzavirus A were fused with P3X63Ag8.653 myeloma cells.
Genes Expressed	immunoglobulin; monoclonal antibody; against influenzavirus A nucleoprotein
Cellular Products	immunoglobulin; monoclonal antibody; against influenzavirus A nucleoprotein
Comments	Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium	The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10⁵ cells/mL and maintain between 1 x 10⁵ and 1 X 10⁶ cells/mL. Medium Renewal: Every 2 to 3 days
Cryopreservation	Complete growth medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions	Temperature: 37°C
Isotype	IgG2a
Name of Depositor	WU Gerhard
Deposited As	mouse (B cell); mouse (myeloma)
References	Yewdell JW, et al. Expression of influenza A virus internal antigens on the surface of infected P815 cells. J. Immunol. 126: 1814-1819, 1981. PubMed: 7217668 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

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