| 產品名稱 |
H18/7 |
| 商品貨號 |
B164524 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with E-selectin and blocks leukocyte binding to endothelial cells. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human E-selectin (ELAM-1, CD62E) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human E-selectin (ELAM-1, CD62E) |
| Comments |
Animals were immunized with human endothelial cells. Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with E-selectin and blocks leukocyte binding to endothelial cells. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
RPMI 1640 medium, 70%, horse serum, 20%; DMSO, 10% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
Secretes a mouse IgG2a monoclonal antibody that reacts with human E-selectin
(ELAM-1, CD62E). |
| Name of Depositor |
M Gimbrone, Brigham and Women's Hospital |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Bevilacqua MP, et al. Identification of an inducible endothelial-leukocyte adhesion molecule. Proc. Natl. Acad. Sci. USA 84: 9238-9242, 1987. PubMed: 2827173
|
