| 產品名稱 |
H57-597 |
| 商品貨號 |
B164542 |
| Organism |
Cricetulus migratorius (B cell); Mus musculus (myeloma), hamster, Armenian (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with all alpha,beta TCR bearing murine T cells, but does not react with receptors on gamma,delta positive T cells. The antibody can directly activate alpha,beta TCR bearing T cells and can be used to immunoprecipitate the receptor from T cell lysates. |
| Derivation |
Animals were immunized with affinity-purified T cell antigen receptor (TCR) from the murine T cell hybridoma DO-11.10. Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against mouse T cell antigen receptor |
| Cellular Products |
immunoglobulin; monoclonal antibody; against mouse T cell antigen receptor |
| Comments |
Animals were immunized with affinity-purified T cell antigen receptor (TCR) from the murine T cell hybridoma DO-11.10. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with all alpha,beta TCR bearing murine T cells, but does not react with receptors on gamma,delta positive T cells. The antibody can directly activate alpha,beta TCR bearing T cells and can be used to immunoprecipitate the receptor from T cell lysates. |
| Complete Growth Medium |
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
- fetal bovine serum to a final concentration of 10%
- 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere
- 0.05 mM 2-Mercaptoethanol
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG |
| Name of Depositor |
RT Kubo |
| Deposited As |
hamster, Armenian (B cell); mouse (myeloma) |
| References |
Kubo RT, et al. Characterization of a monoclonal antibody which detects all murine alphabeta T cell receptors. J. Immunol. 142: 2736-2742, 1989. PubMed: 2467936
White J, et al. The V beta-specific superantigen staphylococcal enterotoxin B: stimulation of mature T cells and clonal deletion in neonatal mice. Cell 56: 27-35, 1989. PubMed: 2521300
Itohara S, et al. Monoclonal antibodies specific to native murine T-cell receptor gamma delta: analysis of gamma delta T cells during thymic ontogeny and in peripheral lymphoid organs. Proc. Natl. Acad. Sci. USA 86: 5094-5098, 1989. PubMed: 2787028
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