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HEC-1-A

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編號(hào):B164586

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產(chǎn)品名稱	HEC-1-A
商品貨號(hào)	B164586
Organism	Homo sapiens, human
Tissue	uterus; endometrium
Product Format	frozen
Morphology	epithelial
Culture Properties	adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	adenocarcinoma
Age	71 years
Gender	female
Karyotype	hypodiploid to hyperdiploid, modal number = 47 with large metacentric marker
Derivation	This line and a substrain HEC-1-B (ATCC HTB-113) were isolated in 1968 by H. Kuramoto and associates from a patient with stage IA endometrial cancer.
Clinical Data	female
Antigen Expression	Blood Type B; Rh+
Receptor Expression	Receptor expression: platelet activating factor (PAF)
Oncogene	c-fos +
Genes Expressed	Oncogenes: c-fos +
Tumorigenic	Yes
Effects	Yes, in nude mice (The cells form moderately well differentiated adenocarcinomas consistent with endometrial carcinoma (grade II).) Yes, in the cheek pouch of cortisone treated hamsters (The cells form typical papillary adenomas.)
Comments	PAF induces increased expression of c-fos.
Complete Growth Medium	The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended Medium Renewal: Every 2 to 3 days
Cryopreservation	Culture medium, 95%; DMSO, 5%
Culture Conditions	Atmosphere: air, 95%; carbon dioxide (CO₂), 5% Temperature: 37°C
Isoenzymes	AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 1 PGM1, 1 PGM3, 1-2
Name of Depositor	H Kuramoto
Deposited As	Homo sapiens
References	Kuramoto H. Studies of the growth and cytogenetic properties of human endometrial adenocarcinoma in culture and its development into an established line. Acta Obstet. Gynaecol. Jpn. 19: 47-58, 1972. PubMed: 4678779 Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Presta M, et al. Modulation of plasminogen activator activity in human endometrial adenocarcinoma cells by basic fibroblast growth factor and transforming growth factor beta. Cancer Res. 48: 6384-6389, 1988. PubMed: 3263185 Maggi M, et al. Platelet-activating factor mediates an autocrine proliferative loop in the endometrial adenocarcinoma cell line HEC-1A. Cancer Res. 54: 4777-4784, 1994. PubMed: 7520361 Kuramoto H, et al. Establishment of a cell line of human endometrial adenocarcinoma in vitro. Am. J. Obstet. Gynecol. 114: 1012-1019, 1972. PubMed: 4673779 Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105 The cells form moderately well differentiated adenocarcinomas consistent with endometrial carcinoma (grade II). The cells form typical papillary adenomas.

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