| 產品名稱 |
HECA-452 |
| 商品貨號 |
B164588 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Tissue |
spleen |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain |
Strain: Wistar |
| Applications |
The antibody reacts with an antigen on skin homing memory T cells. It binds a T cell carbohydrate ligand for vascular E-selectin. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human cutaneous lymphocyte antigen (CLA) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human cutaneous lymphocyte antigen (CLA) |
| Comments |
The antibody reacts with an antigen on skin homing memory T cells. It binds a T cell carbohydrate ligand for vascular E-selectin. The antibody also recognizes sialyl Lewis x and sialyl Lewis a antigens and other carbohydrate ligands for human E-selectin. |
| Complete Growth Medium |
RPMI 1640 medium with 0.05 mM 2-mercaptoethanol and non essential amino acids, 90%; fetal bovine serum, 10%
|
| Subculturing |
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml. Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: culture medium, 92.5%; DMSO, 7.5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
rat IgM |
| Name of Depositor |
Stanford University |
| Deposited As |
rat (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Butcher EC, Picker LJ. Neutrophil LECAM-1 as indicator of neutrophil activation. US Patent 5,316,913 dated May 31 1994
Duijvestijn AM, et al. High endothelial differentiation in human lymphoid and inflammatory tissues defined by monoclonal antibody HECA-452. Am. J. Pathol. 130: 147-155, 1988. PubMed: 3276207
Berg EL, et al. The cutaneous lymphocyte antigen is a skin lymphocyte homing receptor for the vascular lectin endothelial cell-leukocyte adhesion molecule 1. J. Exp. Med. 174: 1461-1466, 1991. PubMed: 1720810
The antibody reacts with a 200-kd cell-surface glycoprotein present on a subset of peripheral blood T cells, including both CD4+ and CD8+ T cells.
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