| 產品名稱 |
HGH-B |
| 商品貨號 |
B164607 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with mouse myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human growth hormone (hGH) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human growth hormone (hGH) |
| Tumorigenic |
Yes |
| Effects |
Yes, forms ascites in BALB/c mice |
| Comments |
Animals were immunized with hGH. Spleen cells were fused with mouse myeloma cells. |
| Complete Growth Medium |
Culture Medium: Dulbecco's modified Eagle's medium (containing: 2 mM
L-glutamine and 1 mM sodium pyruvate) with 4.5 g/L glucose, 90%; fetal bovine
serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Isotype |
IgG1; kappa light chain |
| Name of Depositor |
Genentech, Inc. |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Carlsson LM, et al. Ligand-mediated immunofunctional hormone binding protein assay method. US Patent 5,210,017 dated May 11 1993
|
