Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The hybridoma cell line HIL12R1.2B10 [HIL12R1.2B10.C5.D11] was established by David Presky and Lisa Mondini-Minetti in 1994 and subcloned twice.
The antibody is a useful tool for detection of huIL-12Rbeta1 by flow cytometry, Western Blot analysis and immunoprecipitation.
Derivation
The hybridoma cell line HIL12R1.2B10 [HIL12R1.2B10.C5.D11] was established by David Presky and Lisa Mondini-Minetti in 1994 and subcloned twice.
Clinical Data
HIL12R1.2B10 was formed by the fusion of SP2/0 mouse myeloma cells with splenocytes from an adult female Lewis rat inoculated with transfected COS-7 cells expressing huIL-12Rbeta1.
Genes Expressed
immunoglobulin; monoclonal antibody; against human interleukin 12 (IL-12) receptor beta1 (huIL-12Rbeta1) subunit
Cellular Products
immunoglobulin; monoclonal antibody; against human interleukin 12 (IL-12) receptor beta1 (huIL-12Rbeta1) subunit
Comments
The hybridoma cell line HIL12R1.2B10 [HIL12R1.2B10.C5.D11] was established by David Presky and Lisa Mondini-Minetti in 1994 and subcloned twice.
The antibody is a useful tool for detection of huIL-12Rbeta1 by flow cytometry, Western Blot analysis and immunoprecipitation.
HIL12R1.2B10 was formed by the fusion of SP2/0 mouse myeloma cells with splenocytes from an adult female Lewis rat inoculated with transfected COS-7 cells expressing huIL-12Rbeta1.
Complete Growth Medium
Iscove's Modified Dulbecco's Medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.05 mM 2-mercaptoethanol and 100 U/ml recombinant human IL-6, 90%; fetal bovine serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Alternately, cultures can be established by centrifugation with subsequent resuspension in fresh culture medium.
Establish new cultures at 2 X 10 exp5 viable cells/ml and maintain at between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Culture Conditions
Temperature: 37.0°C
Isotype
IgG2a
Name of Depositor
DH Presky
Year of Origin
1994
References
Wu CY, et al. Biological function and distribution of human interleukin-12 receptor beta chain. Eur. J. Immunol. 26: 345-350, 1996. PubMed: 8617302
