| Applications |
The antibody reacts with human IgG, gamma heavy chain, F(ab') and Fab fragments but not with Fc fragment or rabbit IgG. The antibody from this cell line was selected as a reference reagent of the WHO Collaborating Center at the Centers for Disease Control for Human Immunoglobulin Subclasses. Tested and found negative for ectromelia virus (mousepox). |
| Comments |
Animals were immunized with purified human myeloma protein. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with human IgG, gamma heavy chain, F(ab') and Fab fragments but not with Fc fragment or rabbit IgG. The reactivity is comparable to that of conventional rabbit anti human Fd reagents in radioimmunoassays for rheumatoid factor (RF). The antibody from this cell line was selected as a reference reagent of the WHO Collaborating Center at the Centers for Disease Control for Human Immunoglobulin Subclasses. Tested and found negative for ectromelia virus (mousepox). |
| References |
Reimer CB, et al. Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes. Hybridoma 3: 263-275, 1984. PubMed: 6209201
Jefferis R, et al. Evaluation of monoclonal antibodies having specificity for human IgG sub-classes: Results of an IUIS/WHO collaborative study. Immunol. Lett. 10: 223-252, 1985. PubMed: 3899923
McDougal JS, et al. Immunoassay for IgG rheumatoid factor with a murine monoclonal anti-Fd antibody. J. Lab. Clin. Med. 106: 80-87, 1985. PubMed: 4009026
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
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