| 產(chǎn)品名稱 |
HPAC |
| 商品貨號(hào) |
B164644 |
| Organism |
Homo sapiens, human |
| Tissue |
pancreas |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
adenocarcinoma |
| Age |
64 years |
| Gender |
female |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
modal number = 61 |
| Derivation |
HPAC is a pancreatic adenocarcinoma epithelial cell line derived in 1985 from a nude mouse xenograft of a primary tumor removed from the head of the pancreas of a woman with moderate to well differentiated pancreatic adenocarcinoma of ductal origin. |
| Clinical Data |
64 years
female
Caucasian |
| Receptor Expression |
epidermal growth factor (EGF), expressed glucocorticoid, expressed epidermal growth factor (EGF); glucocorticoid |
| Genes Expressed |
HPAC cells are positive for keratin and negative for vimentin and chromogranin A. |
| Tumorigenic |
Yes |
| Effects |
Yes, the cells form tumors in athymic nude mice at the site of inoculation which are histologically similar to the tumor of origin Yes, the cells have a colony forming efficiency of 64% on plastic and 3.2% in agarose. |
| Comments |
HPAC proliferation is stimulated by insulin, insulin-like growth factor I (IGF-I), epidermal growth factor (EGF), and transforming growth factor alpha (TGF alpha).
Cell growth is suppressed by dexamethasone and other glucocorticoids.
Crude HPAC cell exacts contained significant concentrations of tumor-associated antigens CEA, CA 125, and CA 19-9.
In culture, HPAC cells form monolayers of morphologically heterogenous polar epithelial cells.
HPAC is the first reported human pancreatic adenocarcinoma cell line to express a functional glucocorticoid receptor.
Immunohistochemical staining revealed that HPAC cells express the pancreatic ductal epithelium marker DU-PAN-2 as well as antigens recognized by the monoclonal antibodies HMFG1 and AUA1. |
| Complete Growth Medium |
A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES and 0.5 mM sodium pyruvate supplemented with 0.002 mg/ml insulin, 0.005 mg/ml transferrin, 40 ng/ml hydrocortisone, 10 ng/ml epidermal growth factor and 5% fetal bovine serum
|
| Subculturing |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C
Subculture Ratio: 1:3 to 1:6, every 6 to 8 days.
Medium Renewal: 2 to 3 times a week.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C
Atmosphere: Air, 95%; carbon dioxide (CO2), 5% |
| STR Profile |
Amelogenin: X CSF1PO: 13 D13S317: 11 D16S539: 9,10 D5S818: 12 D7S820: 10,12 THO1: 9.3 TPOX: 10,11 vWA: 15,17 |
| Population Doubling Time |
41 hrs |
| Name of Depositor |
WR Gower |
| Deposited As |
Homo sapiens |
| Year of Origin |
1985 |
| References |
Gower WR Jr., et al. HPAC, a new human glucocorticoid-sensitive pancreatic ductal adenocarcinoma cell line. In Vitro Cell. Dev. Biol. 30A: 151-161, 1994.
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