| 產品名稱 |
Hs 294T |
| 商品貨號 |
B164668 |
| Organism |
Homo sapiens, human |
| Tissue |
skin; derived from metastatic site: lymph node |
| Cell Type |
Melanoma |
| Product Format |
frozen |
| Morphology |
mixed stellate and polygonal |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
melanoma |
| Age |
56 years |
| Gender |
male |
| Ethnicity |
Caucasian |
| Karyotype |
(P26) heteroploid with modal number = 59; Y chromosomes present |
| Derivation |
ATCC HTB-140 (Hs 294T) and ATCC CRL-7898 (A101D) were isolated from the same donor tissue.
|
| Clinical Data |
56 years
Caucasian
male |
| Receptor Expression |
Nerve growth factor (NGF); interferon |
| Tumorigenic |
Yes |
| Effects |
Yes, in immunosuppressed NAMRU mice |
| Comments |
The proliferative rate of the cell line is enhanced by addition of retinoic acid.
Responds to mitogenic stimulation by phytohemagglutinin and forms colonies in soft agar medium or on contact inhibited monolayers. The melanoma cells have also been shown to be responsive to the anti proliferative activity of interferon.
Results from a competitive binding study indicated that Hs 294T cells possess at least two types of interferon (IFN) receptors, one which binds IFN alpha and IFN beta and another which binds IFN gamma.
|
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let the culture sit at 37°C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
|
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| STR Profile |
Amelogenin: X
CSF1PO: 11
D13S317: 11,12
D16S539: 11,12
D5S818: 12
D7S820: 10
TH01: 8,9.3
TPOX: 8,11
vWA: 16,18
|
| Isoenzymes |
AK-1, 1 ES-D, 1 G6PD, B Me-2, 2 PGM1, 1-2 PGM3, 1 |
| Name of Depositor |
RB Owens |
| Deposited As |
Homo sapiens |
| References |
Czarniecki CW, et al. Synergistic antiviral and anti-proliferative activities of Escherichia coli-derived human alpha, beta, and gamma interferons. J. Virol. 49: 490-496, 1984. PubMed: 6319748
Creasey AA, et al. Role of G0-G1 arrest in the inhibition of tumor cell growth by interferon. Proc. Natl. Acad. Sci. USA 77: 1471-1475, 1980. PubMed: 6154934
Helson L. Phytohemagglutinin effects on cultured human neural crest tumors. In Vitro 15: 565-568, 1979. PubMed: 511204
Fabricant RN, et al. Nerve growth factor receptors on human melanoma cells in culture. Proc. Natl. Acad. Sci. USA 74: 565-569, 1977. PubMed: 265522
Creasey AA, et al. Biological properties of human melanoma cells in culture. In Vitro 15: 342-350, 1979. PubMed: 478563
Lotan R. Different susceptibilities of human melanoma and breast carcinoma cell lines to retinoic acid-induced growth inhibition. Cancer Res. 39: 1014-1019, 1979. PubMed: 427741
|
| Cross References |
Nucleotide (GenBank) :
U02329
Human clone ndf294 neu differentiation factor mRNA, partial cds.
|
