| 產(chǎn)品名稱(chēng) |
Hs 700T |
| 商品貨號(hào) |
B164717 |
| Organism |
Homo sapiens, human |
| Tissue |
derived from metastatic site: pelvis |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
adenocarcinoma |
| Age |
61 years |
| Gender |
male |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
modal number = 58 to 59; range = 55 to 107.
The cell line is aneuploid human male, with most chromosome counts in the hypertetraploid range. A substantial (30%) population of hyperdiploid to hypotriploid cells is present. In this population, normal chromosome N7 is absent, while chromosomes N3, N4, N8, N18, and N21 are clearly under-represented with respect to the copy numbers of other normal chromosomes. Chromosomes N2 and N20 tend to be over-represented, while modal number = 58 to 59; range = 55 to 107.
Chromosome N15 is clearly so. Twelve marker chromosomes, some with modifications, are present. A double-minute chromosome was seen in one of 32. |
| Derivation |
Hs 700T was established by R. Owens, et al. at the Naval Biosciences Laboratory from an adenocarcinoma metastatic to the pelvis of a 61 year old male Caucasian.
|
| Clinical Data |
male
Caucasian
61 years |
| Tumorigenic |
Yes |
| Effects |
Yes, in immunosuppressed mice (The cells form well differentiated adenocarcinomas.) |
| Comments |
The mucin producing, primary tumor was thought to have originated from the intestines or pancreas. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: 1:2 to 1:4
Medium Renewal: Every 2 to 3 days
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a Manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
|
| Cryopreservation |
Complete growth medium supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
|
| Culture Conditions |
Temperature: 37°C
Atmosphere: 5% CO2 |
| STR Profile |
Amelogenin: X,Y CSF1PO: 10 D13S317: 8 D16S539: 10,13 D5S818: 11, 12 D7S820: 9, 11 THO1: 9.3 TPOX: 8, 12 vWA: 18, 19 |
| Isoenzymes |
AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 0 PGM1, 1 PGM3, 1 |
| Name of Depositor |
RB Owens |
| Deposited As |
Homo sapiens |
| References |
Owens RB, et al. Epithelial cell cultures from normal and cancerous human tissues. J. Natl. Cancer Inst. 56: 843-849, 1976. PubMed: 176412
Smith HS. In vitro properties of epithelial cell lines established from human carcinomas and nonmalignant tissue. J. Natl. Cancer Inst. 62: 225-230, 1979. PubMed: 283258
Gershwin ME, et al. Immunobiology of heterotransplanted human tumors in nude mice. J. Natl. Cancer Inst. 58: 1455-1463, 1977. PubMed: 857033
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
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