波多野结衣毛片-乳色吐息在线观看-国产伦子伦对白视频-性做久久久-狠狠干2019-黄色裸体片-美女无遮挡免费网站-国产91熟女高潮一区二区-懂色av蜜臀av粉嫩av分享-小h片在线观看-台湾佬在线-日韩激情在线播放-欧日韩不卡在线视频-波多野结衣中文字幕一区-天天操夜夜草

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > Hs 856.T
最近瀏覽歷史
聯系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮海區莊市街道興莊路9號
  • 創e慧谷42號樓B幢401室
Hs 856.T
Hs 856.T
規格:
貨期:
編號:B164752
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 Hs 856.T
商品貨號 B164752
Organism Homo sapiens, human
Tissue connective tissue
Product Format frozen
Morphology mixed
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease histiocytoma
Age 16 years
Gender female
Ethnicity Black
Karyotype modal number = 46; range = 45 to 46
Images ATCC CRL-7593 Cell Micrograph
Clinical Data
female
Black
16 years
Comments
Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty.
Please see the NBL Repository description.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
  • Remove and discard culture medium.
  • Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  • Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
  • Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting
  • Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
  • Incubate cultures at 37C.
Subcultivation ratio: A subcultivation ratio of 1:3 to 1:6 is recommended.
Medium renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 7, 8
D13S317: 11
D16S539: 11, 12
D5S818: 8, 11
D7S820: 11
THO1: 7, 9
TPOX: 7, 10
vWA: 15, 18
梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
主站蜘蛛池模板: 中文字幕免费在线视频 | а√在线中文网新版地址在线 | 亚洲日日骚 | 精品一区二区无码 | 少妇高潮一区二区三区 | 美女88av | 久久亚洲av无码精品色午夜麻豆 | 国产精品一区二区在线播放 | 99久久一区二区 | 大地资源高清播放在线观看 | 日韩激情久久 | www国产com | 亚洲清色 | 亚洲一区二区三 | 很嫩很紧直喷白浆h | 能直接看的av网站 | 丁香六月激情综合 | 亚洲精品v日韩精品 | 亚洲天堂小说 | 韩日精品在线 | www国产91| 国产综合久久久 | 亚洲色图19p | 亚洲国产第一区 | 色汉综合 | 国模私拍一区二区三区 | 日韩中文久久 | 综合色亚洲 | 国产精品视频免费网站 | 精品久久亚洲 | 波多野结衣免费看 | 日本美女一区二区 | 曰韩一级片| 国产精品久久久久久久av | 国产在线视频网站 | 亚洲色在线视频 | 欧美成人毛片 | 97福利影院 | 婷婷综合 | 久操新在线 | 毛片入口| 亚洲一二三精品 | 久久免费在线视频 | 99国产精品久久久久 | 夜色快播 | 苍井空张开腿实干12次 | 亚洲最大成人网站 | 亚洲三级黄色片 | 91福利在线播放 | 亚洲综合在线观看视频 | 亚洲系列在线观看 | 少妇高潮网站 | a视频免费看 | 婷婷六月天 | 欧亚成人av | 日本成人激情视频 | 香蕉视频免费在线看 | 最近中文字幕mv免费高清在线 | 日韩电影中文字幕 | 亚洲国产精品久久久久 | 国产精品资源网 | 在线h片 | 亚洲免费网址 | 国产极品尤物 | 老熟女高潮喷水了 | 奇米影视久久 | 99久热 | 熟妇高潮精品一区二区三区 | 在线毛片观看 | 性欧美一区二区三区 | 午夜日韩av| 91爱国产| 亚洲国产中文字幕在线观看 | 涩涩视频免费观看 | 亚洲黄色在线免费观看 | 少妇淫片 | 尹人久久 | 久久影 | 国产乱淫av片免费 | 乡村乱淫 | 中文字幕日韩av | 韩国伦理片在线观看 | 91视频网| 啪啪免费av | 日韩精品专区 | 拔插拔插华人 | 久爱视频在线观看 | 女人被灌满精子 | 日韩在线导航 | 欧美 日韩 国产一区 | 蜜桃av一区 | 韩国三级hd中文字幕叫床浴室 | 中文字幕久热 | 欧美第一页| 久久久久久久久99精品 | 女人扒开腿免费视频app | 5个黑人躁我一个视频 | 黑人干亚洲女人 | 亚洲天堂视频在线观看 |