| 產品名稱 |
HS-21 (subclone 1H3) |
| 商品貨號 |
B164785 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with an antigen on the acrosomal cap of mammalian sperm. It is useful in immunofluorescent assays to evaluate acrosomal status in sperm samples. |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against mammalian sperm acrosomal vesicle |
| Cellular Products |
immunoglobulin; monoclonal antibody; against mammalian sperm acrosomal vesicle |
| Comments |
Animals were immunized with human sperm. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with an antigen on the acrosomal cap of mammalian sperm. It is useful in immunofluorescent assays to evaluate acrosomal status in sperm samples. |
| Complete Growth Medium |
CULTURE MEDIUM: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%;
fetal bovine serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Isotype |
IgG1 |
| Name of Depositor |
KB Bechtol |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Wolf DP, et al. Acrosomal status evaluation in human ejaculated sperm with monoclonal antibodies. Biol. Reprod. 32: 1157-1162, 1985. PubMed: 3926014
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