| 產(chǎn)品名稱 |
Hs27 |
| 商品貨號(hào) |
B164786 |
| Organism |
Homo sapiens, human |
| Tissue |
skin, foreskin |
| Cell Type |
fibroblast |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
normal |
| Age |
newborn |
| Gender |
male |
| Ethnicity |
Black |
| Storage Conditions |
liquid nitrogen vapor phase |
| Clinical Data |
Black male newborn |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X,Y CSF1PO: 10,12 D13S317: 12,14 D16S539: 11,13 D5S818: 12 D7S820: 9,11 THO1: 7,8 TPOX: 6,8 vWA: 18 |
| Isoenzymes |
G6PD, A |
| Name of Depositor |
RB Owens |
| Deposited As |
Homo sapiens |
| References |
Suss-Toby E, et al. Toxoplasma invasion: the parasitophorous vacuole is formed from host cell plasma membrane and pinches off via a fission pore. Proc. Natl. Acad. Sci. USA 93: 8413-8418, 1996. PubMed: 8710885
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