| 產品名稱 |
HT |
| 商品貨號 |
B164792 |
| Organism |
Homo sapiens, human |
| Tissue |
ascites |
| Cell Type |
B lymphoblast |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
diffuse mixed lymphoma |
| Age |
70 years |
| Gender |
male |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
HT is a human B cell lymphoma cell line originated in 1983 by Walter J. Urba and Dan L. Longo. |
| Clinical Data |
70 years Caucasian male |
| Antigen Expression |
CD19 +; CD20 +; CD21 +; CD22 +; Hle-1 +; HLA DQ +; HLA DR +; CD25 -; T cell receptor (TCR) - |
| Genes Expressed |
immunoglobulin; kappa light chain |
| Cellular Products |
immunoglobulin; kappa light chain |
| Comments |
HT cells express mRNA for both IgM and kappa and express low levels of kappa on the cell surface.
Exposure of HT cells to protein kinase C activating phorbol esters such as PMA and PdBu induced profound growth inhibition.
HT cells have been reported to be Epstein-Barr virus genome negative. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL. Maintain cultures at a cell concentration between 5 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 2 x 106 cells/mL.
Medium Renewal: 2 to 3 times a week. |
| Cryopreservation |
Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X,Y CSF1PO: 10 D13S317: 13,14 D16S539: 11,13 D5S818: 11,13 D7S820: 8,10 THO1: 6,7 TPOX: 11 vWA: 17,18 |
| Isotype |
kappa |
| Name of Depositor |
M Beckwith |
| Deposited As |
Homo sapiens |
| Year of Origin |
1983 |
| References |
Beckwith M, et al. Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723
|
