| 產品名稱 | HuT 78 |
|---|---|
| 商品貨號 | B164807 |
| Organism | Homo sapiens, human |
| Cell Type | cutaneous T lymphocyte |
| Product Format | frozen |
| Morphology | lymphoblast |
| Culture Properties | suspension |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | Sezary Syndrome |
| Age | 53 years adult |
| Gender | male |
| Ethnicity | Caucasian |
| Applications | This cell line is a suitable transfection host. |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Derivation | H9 (ATCC HTB-176) is a clonal derivative of HuT 78 Ref   Mann DL, et al. Origin of the HIV-susceptible human CD4+ cell line H9. AIDS Res. Hum. Retroviruses 5: 253-255, 1989. PubMed: 2567177. |
| Clinical Data | 53 years adult Caucasian male |
| Antigen Expression | Antigen expression: CD4; Homo sapiens Ref Gazdar AF, et al. Mitogen requirements for the in vitro propagation of cutaneous T-cell lymphomas. Blood 55: 409-417, 1980. PubMed: 6244013 |
| Receptor Expression | Receptor expression: interleukin 2 (IL-2), expressed Ref Gootenberg JE, et al. Human cutaneous T cell lymphoma and leukemia cell lines produce and respond to T cell growth factor. J. Exp. Med. 154: 1403-1418, 1981. PubMed: 6975346 |
| Genes Expressed | interleukin 2, tumor necrosis factor alpha (TNF alpha), CD4; Homo sapiens |
| Tumorigenic | Yes |
| Effects | Yes, in nude mice when injected intracranially |
| Comments | |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
|
| Subculturing | Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 2 X 105 viable cells/ml. Maintain cultures at cell concentrations between 5 X 104 and 8 X 105 viable cells/mL. Maintain cell density at less than 1 X 106 cells/mL.
Medium Renewal: Two to three times weekly |
| Cryopreservation | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37°C |
| STR Profile | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 8,12 D16S539: 11,12 D5S818: 11,12 D7S820: 8,11 THO1: 8,9 TPOX: 8,9 vWA: 14,15 |
| Population Doubling Time | about 65 hours |
| Name of Depositor | AF Gazdar |
| Deposited As | Homo sapiens |
| References | Gootenberg JE, et al. Human cutaneous T cell lymphoma and leukemia cell lines produce and respond to T cell growth factor. J. Exp. Med. 154: 1403-1418, 1981. PubMed: 6975346 Mann DL, et al. Origin of the HIV-susceptible human CD4+ cell line H9. AIDS Res. Hum. Retroviruses 5: 253-255, 1989. PubMed: 2567177 Gazdar AF, et al. Mitogen requirements for the in vitro propagation of cutaneous T-cell lymphomas. Blood 55: 409-417, 1980. PubMed: 6244013 Chen TR. Karyotypic derivation of H9 cell line expressing human immunodeficiency virus susceptibility. J. Natl. Cancer Inst. 84: 1922-1926, 1992. PubMed: 1460674 O'Connell MA, et al. Cellular proliferation and activation of NF kappa B are induced by autocrine production of tumor necrosis factor alpha in the human T lymphoma line HuT 78. J. Biol. Chem. 270: 7399-7404, 1995. PubMed: 7706285 Hu SX, et al. Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene expression. Cancer Res. 57: 3339-3343, 1997. PubMed: 9269991 Kolanus W, et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128 Bloom TJ, Beavo JA. Identification and tissue-specific expression of PDE7 phosphodiesterase splice variants. Proc. Natl. Acad. Sci. USA 93: 14188-14192, 1996. PubMed: 8943082 Hut 78 was derived from peripheral blood of a patient with Sezary syndrome. The line has the properties of a mature human T cell with helper/inducer activity. It releases IL-2, and has receptors for IL-2. The growth rate is stimulated by IL-2. TNF alpha is an autocrine growth factor for Hut 78. |
| Cross References | Nucleotide (GenBank) : X95325 H.sapiens mRNA for DNA binding protein A variant. Nucleotide (GenBank) : NM_003651 Homo sapiens cold shock domain protein A (CSDA), mRNA. Nucleotide (GenBank) : NM_006231 Homo sapiens polymerase (DNA directed), epsilon (POLE), mRNA. Nucleotide (GenBank) : L09561 Homo sapiens DNA polymerase epsilon, catalytic polypeptide mRNA, complete cds. |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
