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編號:B164829

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產品名稱	IEC-6 [IEC6]
商品貨號	B164829
Organism	Rattus norvegicus, rat
Tissue	small intestine/epithelium
Product Format	frozen
Morphology	epithelial
Culture Properties	adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	normal
Gender	male
Applications	This cell line is a suitable transfection host.
Storage Conditions	liquid nitrogen vapor phase
Clinical Data	male
Comments	Cell growth is inhibited by cortisol. The cells express antigens characteristic of intestinal epithelia. The depositor notes that the cells maintain a constant growth rate and cell morphology until approximately the 20th passage, thereafter, either cell growth rapidly declines or morphological changes are observed.
Complete Growth Medium	Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.1 Unit/ml bovine insulin, 90%; fetal bovine serum, 10%
Subculturing	Remove medium, and rinse the monolayer with fresh 0.25% trypsin, 0.53 mM EDTA solution. Remove the trypsin, add fresh trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: Twice per week
Cryopreservation	Freeze medium: culture medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
Culture Conditions	Temperature: 37°C
Name of Depositor	A Quaroni
Deposited As	Rattus sp.
Passage History	The depositor notes that the cells maintain a constant growth rate and cell morphology until approximately the 20th passage, thereafter, either cell growth rapidly declines or morphological changes are observed.
References	Quaroni A, et al. Fibronectin synthesis by epithelial crypt cells of rat small intestine. Proc. Natl. Acad. Sci. USA 75: 5548-5552, 1978. PubMed: 103096 Quaroni A, et al. Epithelioid cell cultures from rat small intestine. Characterization by morphologic and immunologic criteria. J. Cell Biol. 80: 248-265, 1979. PubMed: 88453 Quaroni A, et al. Keratin expression in rat intestinal crypt and villus cells. J. Biol. Chem. 266: 11923-11931, 1991. PubMed: 1711043 Dignass AU, Podolsky DK. Interleukin 2 modulates intestinal epithelial cell function in vitro. Exp. Cell Res. 225: 422-429, 1996. PubMed: 8660931 Weiser MM, Quaroni A. A vitamin D-related inhibition of growth of an epithelioid cell line derived from rat small intestine. Biochem. Biophys. Res. Commun. 90: 788-793, 1979. PubMed: 508345 Jakobs ES, et al. Expression of sodium-linked nucleoside transport activity in monolayer cultures of IEC-6 intestinal epithelial cells. J. Biol. Chem. 265: 22210-22216, 1990. PubMed: 2266122 Conteas CN, Majumdar AP. The effects of gastrin, epidermal growth factor, and somatostatin on DNA synthesis in a small intestinal crypt cell line (IEC-6). Proc. Soc. Exp. Biol. Med. 184: 307-311, 1987. PubMed: 2881310
Cross References	Nucleotide (GenBank) : Z46374 R.norvegicus mRNA for c-met. Nucleotide (GenBank) : AF008587 Rattus norvegicus hereditary hemochromatosis protein homolog (RT1-CAFe) mRNA, partial cds.

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