| 產品名稱 |
IGEL a2 [15.3] |
| 商品貨號 |
B164834 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions |
liquid nitrogen vapor temperature |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against 2,4,6-trinitrophenyl (TNP) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against 2,4,6-trinitrophenyl (TNP) |
| Comments |
Animals were immunized with TNP-Asc. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml ay maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml. Do not allow cell concentration to exceed 2 X 10 exp6 cells/ml. Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium 90%; DMSO, 10% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgE (Iga haplotype) |
| Name of Depositor |
M Wabl |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Rudolph AK, et al. Thirteen hybridomas secreting hapten-specific immunoglobulin E from mice with Iga or Igb heavy chain haplotype. Eur. J. Immunol. 11: 527-529, 1981. PubMed: 6790293
|
| Cross References |
Nucleotide (GenBank) :
X65774
M.musculus DNA for IgE antibody light chain (VJ).
Nucleotide (GenBank) :
X65772
M.musculus DNA for IgE antibody heavy chain (VDJ).
|
