| 產品名稱 |
IgG-1C6 |
| 商品貨號 |
B164839 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Animals were immunized with a bacterially produced fusion protein encoding 6 consecutive histidines followed by amino acids 833 to 1141 of human SREBP. The antibody recognizes the carboxy terminal portion of human SREBP-2, and can be used in immunoblotting assays to detect carboxy terminal fragments of SREBP-2. |
| Derivation |
Animals were immunized with a bacterially produced fusion protein encoding 6 consecutive histidines followed by amino acids 833 to 1141 of human SREBP. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human sterol regulatory element binding protein 2 (SREBP 2, SREBP-2) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human sterol regulatory element binding protein 2 (SREBP 2, SREBP-2) |
| Tumorigenic |
Yes |
| Effects |
Yes, in BALB/c mice |
| Comments |
Animals were immunized with a bacterially produced fusion protein encoding 6 consecutive histidines followed by amino acids 833 to 1141 of human SREBP. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody recognizes the carboxy terminal portion of human SREBP-2, and can be used in immunoblotting assays to detect carboxy terminal fragments of SREBP-2. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG1 |
| Name of Depositor |
JL Goldstein, YK Ho |
| Deposited As |
mouse (B cell); mouse (myeloma) |
