| Comments |
Animals were immunized against the GST-human SREBP-2 fusion protein (amino acids 48 to 403). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody recognizes both the precursor and mature forms of SREBP-2 in cultured human cells, and can be used in immunoblotting and ELISA assays to study SREBP-2 in cultured human cells grown under different conditions. |
| References |
Janowski BA, et al. The hypocholesterolemic agent LY295427 reverses suppression of sterol regulatory element-binding protein processing mediated by oxysterols. J. Biol. Chem. 48: 45408-45416, 2001. PubMed: 11577112
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Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.
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