| 產(chǎn)品名稱 |
IgG-4A4 |
| 商品貨號(hào) |
B164842 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Animals were immunized with a synthetic peptide corresponding to the carboxy terminal 14 amino acids of human low density lipoprotein receptor. The antibody reacts with LDL receptor and can be used to anchor the receptor for solid phase assays. |
| Derivation |
Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human low density lipoprotein (LDL) receptor |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human low density lipoprotein (LDL) receptor |
| Tumorigenic |
Yes |
| Effects |
Yes, in BALB/c mice |
| Comments |
Animals were immunized with a synthetic peptide corresponding to the carboxy terminal 14 amino acids of human low density lipoprotein receptor. Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with LDL receptor and can be used to anchor the receptor for solid phase assays. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
YK Ho, JL Goldstein |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
van Driel IR, et al. First cysteine-rich repeat in ligand-binding domain of low density lipoprotein binds Ca2+ and monoclonal antibodies, but not lipoproteins. J. Biol. Chem. 262: 17443-17449, 1987. PubMed: 3320043
Van Driel IR, et al. Stoichiometric binding of low density lipoprotein (LDL) and monoclonal antibodies to LDL receptors in a solid phase assay. J. Biol. Chem. 264: 9533-9538, 1989. PubMed: 2722848
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