| 產品名稱 |
IL-A11 |
| 商品貨號 |
B164849 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with a 52000 to 64000 dalton antigen on bovine T cells that is equivalent to human CD4. This is one of a series of hybridomas produced by Dr. |
| Derivation |
Spleen cells were fused with P3X63Ag8 myeloma cells. This is one of a series of hybridomas produced by Dr. W. Ivan Morrison at the International Laboratory for Research on Animal Diseases (ILRAD). |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against bovine CD4 |
| Cellular Products |
immunoglobulin; monoclonal antibody; against bovine CD4 |
| Tumorigenic |
Yes |
| Effects |
Yes, in BALB/c mice |
| Comments |
Animals were immunized with uncloned alloreactive bovine lymphocytes. Spleen cells were fused with P3X63Ag8 myeloma cells. The antibody reacts with a 52000 to 64000 dalton antigen on bovine T cells that is equivalent to human CD4. This is one of a series of hybridomas produced by Dr. W. Ivan Morrison at the International Laboratory for Research on Animal Diseases (ILRAD). |
| Complete Growth Medium |
RPMI 1640 medium with 0.02 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG2a |
| Name of Depositor |
FA Osorio, WI Morrison |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Baldwin CL, et al. Characterization of a subset of bovine T lymphocytes that express BoT4 by monoclonal antibodies and function: Similarity to lymphocytes defined by human T4 and murine L3T4. J. Immunol. 136: 4385-4391, 1986. PubMed: 2423591
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