| Comments |
Animals were immunized with human cerebral white matter from a multiple sclerosis (MS) patient. Spleen cells were fused with NS-1 myeloma cells. The antibody recognizes an intracellular protein antigen (MW 30000) expressed by human and rat astrocytes and other specialized glia. (Muller cells of the retina, Bergmann fibers of the cerebellar cortex, tanycytes of the hypothalamus and ciliated ependymal cells) in the central nervous system (CNS). This marker is distinct from glial fibrillary acidic protein (GFAP) and vimentin. Astrocytes in the vicinity of a CNS lesion show enhanced staining for J1-31 antigen. J1-31 monoclonal antibody recognizes a protein whose expression is increased following CNS injury. The antibody is useful for immunofluorescence, immunocytochemistry, and Western blotting procedures. |
| References |
Singh R, et al. A new "marker" protein for astrocytes. Biosci. Rep. 6: 73-80, 1986. PubMed: 2421799
. . Neurosci. Res. Commun. 4: 25031, 1989.
. . Dendron 1: 91-108, 1992.
Predy R, et al. Enhanced expression of a protein antigen (J1-31 antigen, 30 kilodaltons) by reactive astrocytes in lacerated spinal cord. J. Neurosci. Res. 19: 397-404, 1988. PubMed: 3385801
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.
|
