| 產(chǎn)品名稱 |
JAR |
| 商品貨號(hào) |
B164884 |
| Organism |
Homo sapiens, human |
| Tissue |
placenta |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
choriocarcinoma |
| Age |
fetus |
| Gender |
male |
| Ethnicity |
Caucasian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
This is probably a pseudotriploid human cell line with the modal chromosome number of 68, occurring in 24% of cells, but cells with both 69 (22%) and 70 (18%) chromosome counts also occurred frequently. Cells with higher ploidies occurred at 7.0%.
Karyotypes were extremely complex. Consistently there were 20 to 25 marker chromosomes (>30% of total chromosome content) per cell. Most marker chromosomes had complex structural rearrangements, and the origin of chromosome segments of these markers often defied identification. Among the frequently found markers were 8p+, 11p, a large metacentric [?t(3qter--3q12::?--C--?::3q12--3qter)] der(?13)T(1;?13) (p13;?q14), and many others. There was only one normal X chromosome. Normal N3 and N13 were not found. |
| Derivation |
The JAR line was established by R. A. Pattillo and associates directly from a trophoblastic tumor of the placenta. |
| Clinical Data |
Caucasian fetus male |
| Genes Expressed |
estrogen; progesterone; human chorionic gonadotropin (hCG); human chorionic somatomammotropin (placental lactogen); hCG production averages 22.5 ng/mL after reculturing |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.03% (w/v) EDTA solution to remove all traces of serum which contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: Twice per week |
| Cryopreservation |
Freeze medium: Culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile |
Amelogenin: X,Y CSF1PO: 7,10 D13S317: 11 D16S539: 9,10 D5S818: 10,11 D7S820: 10,11 THO1: 6,7 TPOX: 8,11 vWA: 16,18 |
| Isoenzymes |
AK-1, 1 ES-D, 2 G6PD, B GLO-I, 1 PGM1, 1-2 PGM3, 1-2 |
| Name of Depositor |
RA Pattillo |
| Deposited As |
Homo sapiens |
| References |
Pattillo RA, et al. The Jar cell line -- continuous human multihormone production and controls. In Vitro 6: 398-399, 1971.
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