| 產(chǎn)品名稱 |
JAWSII |
| 商品貨號 |
B164885 |
| Organism |
Mus musculus, mouse |
| Tissue |
bone marrow |
| Cell Type |
immature dendritic cell; monocyte |
| Product Format |
frozen |
| Morphology |
monocyte |
| Culture Properties |
mixed, adherent and suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain |
C57BL/6 |
| Storage Conditions |
liquid nitrogen vapor phase |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Complete Growth Medium |
Alpha minimum essential medium with ribonucleosides, deoxyribonucleosides, 4 mM L-glutamine, 1 mM sodium pyruvate and 5 ng/ml murine GM-CSF, 80%; fetal bovine serum, 20%
|
| Subculturing |
Cultures can be maintained by transferring floating cells to a centrifuge tube.
Attached cells may be subcultured using 0.25% trypsin-0.03% EDTA.
Pool cells and centrifuge the cell suspension at 1000 rpm for 10 minutes, resuspend the pellet in fresh medium, aspirate and dispense into new flasks.
Note: This cell line grows very slowly.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: Once per week |
| Cryopreservation |
Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C |
| Name of Depositor |
ZymoGenetics, Inc. |
| U.S. Patent Number |
|
| References |
MacKay VL, Moore EE. Immortalized dendritic cells. US Patent 5,648,219 dated Jul 15 1997
Moore EE. Preparation of immortalized cells. US Patent 5,830,682 dated Nov 3 1998
|
