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JM1

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編號:B164894

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產(chǎn)品名稱	JM1
商品貨號	B164894
Organism	Homo sapiens, human
Tissue	blood
Cell Type	pre-B lymphoblast
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	B cell lymphoma
Storage Conditions	liquid nitrogen vapor phase
Disclosure	This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation	The JM1 cell line was established from cells taken from a patient with immunoblastic B cell lymphoma - leukemia.
Antigen Expression	HLA-DR; Homo sapiens, expressed CD19; Homo sapiens, expressed CD10; Homo sapiens, expressed
Genes Expressed	CD19; Homo sapiens, expressed ,CD10; Homo sapiens, expressed ,HLA-DR; Homo sapiens, expressed
Comments	The cells are negative for CD7, My 7, My 9 and surface IgM.
Complete Growth Medium	The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: 0.05 mM 2-mercaptoethanol fetal bovine serum to a final concentration of 10%
Subculturing	Cultures can be maintained by addition or replacement of fresh medium. Alternatively, cultures may be established by centrifugation. Adherent cells can be dislodged by scraping. Initiate cultures at 2 to 5 x 10⁵ viable cells/mL. Subculture when cell density reaches 1 x 10⁶ cells/mL. Medium Renewal: 2 to 3 times per week
Cryopreservation	Complete culture medium, 95%; DMSO 5%. Cell culture tested DMSO is available as ATCC^? Catalog No. 4-X.
Culture Conditions	Temperature: 37°C Atmosphere: Air, 95%; Carbon dioxide (CO₂), 5%
STR Profile	D5S818: 12 D13S317: 11 D7S820: 8, 10 D16S539: 9 vWA: 17 THO1: 6 TPOX: 10, 11 CSF1PO: 12 Amelogenin: X Y
Name of Depositor	Immunex Corporation
Deposited As	Homo sapiens
U.S. Patent Number	5,976,860
References	Park LS, et al. Heterogeneity in human interleukin-3 receptors. A subclass that binds human granulocyte/macrophage colony stimulating factor. J. Biol. Chem. 264: 54205427, 1989. PubMed: 2647717 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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